PNIPAM-based Smart Surfaces for Cell Sheet Tissue Engineering Thermosensitive polymer, can be used to form a hydrogel. Aqueous polymer solution undergoes a phase transition from a soluble to an insoluble state when the temperature is raised to ca. 32 °C.
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7. Dispersibility of GO Sheets in Swollen GO/PNIPAM IPN Hydrogel. Fig. S6. Confocal Raman image of swollen GO/PNIPAM IPN hydrogel acquired by detecting the intensity of D peak at 1322 cm-1. Wherein, red regions are GO-rich ones, while green regions are where water and PNIPAM microgels are. PNIPAM hydrogels are widely used in research on tissue engineering. Cells harvested from patients can be grown on PNIPAM or PNIPAM-coated substrates to form viable cell sheets, which can then be layered to form a tissue (Cooperstein and Canavan, 2010; Cooperstein et al., 2017).
We found that the hybrid hydrogel sheet started to deform while its surface temperature increased from 22 °C to 29 °C (Fig.S5a) during the irradiation process and it went back to its original shape when it cooled down. Surface temperature of pure and hybrid PNIPAM hydrogel under laser irradiation. In the Poly(N-isopropylacrylamide) (variously abbreviated PNIPA, PNIPAAm, NIPA, PNIPAA or PNIPAm) is a temperature-responsive polymer that was first synthesized in the 1950s.It can be synthesized from N-isopropylacrylamide which is commercially available.